Power Green qPCR Mix with ROX for ABI Roche DNA real time PCR

Cat. No: P2101c, 400 rxn/20μl reaction

P2102c, 2000 rxn/20μl reaction

Power Green qPCR Mix with ROX

Power Green qPCR Mix with ROX P2101c/P2102c

For research use only

Components

* ROX Reference Dye H and ROX Reference Dye L are suitable for fluorescence quantitative PCR instruments requiring high and low concentrations of ROX calibration respectively. It is recommended that 40 μL 50X ROX Reference Dye H or L be premixed into 1ml 2X SYBR Green qPCR Mix. The final working concentration of ROX is 1X.

Storage

This mix can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light. The ROX Reference Dye should be kept at -20°C and protected from light.

Description

Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.

The Hotstart Taq DNA Polymerase in the mix is modified by antibody. When the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This technology helps to reduce non-specific amplification and primer dimer effectively.

The reaction system of this product can be prepared at room temperature without an ice box. The prepared PCR reaction system can be placed at room temperature for 24 hours and the amplification efficiency remains unchanged.

Applications

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

Features

• Compatible with many Real-time systems whether requires ROX reference dye to calibration or not

• Exceptional specificity with dual hot-start mechanism

• Tight reproducibility in Ct values over a broad dynamic range

• Universal instrument compatibility

Table of Instrument Guide

Testing Data:
Power Green qPCR Mix

Cat. No.: P2101/P2102
The Power Green qPCR Mix utilizes an antibody-modified hot-start Taq DNA polymerase to enhance the specificity of the reaction, ensuring the accuracy of gene quantification analysis. Specially optimized, this product allows for the preparation of the reaction system without the need for an ice box, and can be directly prepared at room temperature with components such as PCR Mix, primers, and templates. The prepared PCR reaction system can be kept at room temperature for 24 hours without changing the amplification efficiency, ensuring the stability of the workbench for large-scale testing.

qPCR Amplification Curve (left) and Melting Curve (right)

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

Founded in 2005, located in the Science City of Guangzhou, China, Guangzhou Dongsheng Biotech Co., Ltd (GDSBio) is a high-tech enterprise focusing on R&D, production and sales of high-quality life science products and in vitro diagnostic reagents.

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, NGS library preparation, nucleic acid electrophoresis, nucleic acid purification, etc.It has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.

Supply Mode: retail/bulk/custom/OEM

With strict and perfect standards, we focus on each product to ensure that every product delivered is in perfect condition.

We provide professional and comprehensive diagnostic and medical test solutions. And we work with clinical experts to continuously improve technology in practice so as to provide more accurate test data for clinical diagnosis.

Since 2010, when GDSBio was certified with ISO9001 and ISO13485 standards in sequence, it has opened up overseas markets. Over the past 18 years, our customers have spread all over the world. With stable and excellent quality, the products have won a large number of loyal customers.

GDSBio's international resource links and multi-dimensional biological research methods help it stand at the forefront of the industry. To interpret the world with high-end technology, insight into the biological mystery.

ISO 13485 certificate

ISO 9001 certificate