V5001 One Step RNA Rt qPCR Master Mix with ROX

One-step Probe RT-qPCR Kit

For research use only

Components

a. Contains dNTP/dUTP Mix, Mg²⁺.

b. Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and hot-start Taq DNA Polymerase.

c. Used to correct the fluorescence signal error between holes. 50 × ROX Reference Dye 1 is used for ABI 7900HT/ 7300 real-time PCR System and StepOne Plus; ABI 7500, 7500 Fast real-time PCR System, Stratagene Mx3000P using 50 × ROX Reference Dye 2; Roche, bio-rad Real Time PCR Systems do not have to use ROX.

Storage

This reagent should be kept at -20°C.

Description

The One-step Probe RT-qPCR Kit is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 2 × One-step U⁺ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan^®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

Applications

• Probe gene expression analysis

• Probe Low-copy gene detection

• Probe microarray validation

• Probe gene knockdown validation

Features

• One-step RT-qPCR

• This kit is suitable for fluorescence quantification by probe method

• This kit is compatible with many real-time systems

• Hot-start technology brings high specificity and reproducible amplification

• dUTP/UDG system, effectively prevent PCR product contamination

Product Selection Guide

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

Founded in 2005, located in the Science City of Guangzhou, China, Guangzhou Dongsheng Biotech Co., Ltd (GDSBio) is a high-tech enterprise focusing on R&D, production and sales of high-quality life science products and in vitro diagnostic reagents.

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, NGS library preparation, nucleic acid electrophoresis, nucleic acid purification, etc.It has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.

Supply Mode: retail/bulk/custom/OEM

With strict and perfect standards, we focus on each product to ensure that every product delivered is in perfect condition.

We provide professional and comprehensive diagnostic and medical test solutions. And we work with clinical experts to continuously improve technology in practice so as to provide more accurate test data for clinical diagnosis.

Since 2010, when GDSBio was certified with ISO9001 and ISO13485 standards in sequence, it has opened up overseas markets. Over the past 18 years, our customers have spread all over the world. With stable and excellent quality, the products have won a large number of loyal customers.

GDSBio's international resource links and multi-dimensional biological research methods help it stand at the forefront of the industry. To interpret the world with high-end technology, insight into the biological mystery.

ISO 13485 certificate

ISO 9001 certificate