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GDSBio One Step RT QPCR Master Mix V5009 V5010

Categories One Step RT QPCR Master Mix
Brand Name: GDSBio
Model Number: V5009/V5010
Certification: /
Place of Origin: China
MOQ: 1Bag
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Delivery Time: 8 work days
Packaging Details: small package or bulk distribute or OEM
Cat. No.: V5009/V5010
Specification: 200 rxn /5000 rxn
Appearance: colourless
Application: RNA reverse transcription and quantitative PCR
Activity: Pass
Amplification capability: Good
Logo Printing: With Logo Printing
Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day
Storage Conditions: Store at -20°C
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GDSBio One Step RT QPCR Master Mix V5009 V5010

One-step Probe RT-qPCR Kit V2

High Quality One-Step Probe RT-QPCR Kit V2 V5009/V5010

Cat. No.: V5009,V5010


Components

ComponentV5009 (200 rxn, 30 μl/rxn)V5010 (5000 rxn, 30 μl/rxn)
2X RT-qPCR Mix *760 μl × 438.4 ml × 2
Enzyme Mix b308 μl7.7 ml

a. Contains dNTP/dUTP Mix, Mg2+.

b. Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and hot-start Taq DNA Polymerase.


Storage

This reagent should be kept at -20°C.


Description

The One-step Probe RT-qPCR Kit V2 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Thermostable reverse transcriptase can maintain stable activity at 55°C. The hot-start antibody-modified fast DNA polymerase and optimized buffer system ensure the extremely high sensitivity and specificity of the One Step Probe RT-qPCR Kit V2. The kit is available as a convenient Master Mix. 2 × RT-qPCR Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan®.


Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

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